Phosbind是一种新型的磷酸盐结合标签和功能分子,在中性pH(生理pH)条件下可以特异性结合磷酸离子。Phosbind是一种双环金属络合物(1,3-bis[bis(pyridin-2-ylmethyl) amino]propan-2-olato dizinc(II) complex),是一种选择性的磷酸结合标签,在中性pH的水溶液中,其对苯基磷酸酯二价阴离子(PH-OPO32-)的Kd值为25 nM* (*:在Zn2+的情况下)。
Phosbind Acrylamide提供了一种特异性的电泳过程(锰 (II)- Phosbind SDS-PAGE),可同时对磷酸化蛋白及其对应的非磷酸化形式进行分析。
此产品最适合做的蛋白大小在30 KDa-130 KDa之间
- 1. Haijing Guo, Jen-Hsuan Wei, et al. "Importin α phosphorylation promotes TPX2 activation by GM130 to control astral microtubules and spindle orientation." J Cell Sci. 2021 Feb 19;134(4):jcs258356. PMID:33526712
- 2. Amina Merwa LEKEHAL. "altFUS, the second protein encoded in the FUS gene, inhibits mitochondrial recruitment of PARKIN." Mars, 2021.
- 3. Fan XJ, Wang YL, et al. "NONO phase separation enhances DNA damage repair by accelerating nuclear EGFR-induced DNA-PK activation." Am J Cancer Res. 2021 Jun 15;11(6):2838-2852. PMID:34249431
- 4. Wirth M, Mouilleron S, et al. "Phosphorylation of the LIR Domain of SCOC Modulates ATG8 Binding Affinity and Specificity." J Mol Biol. 2021 Jun 25;433(13):166987. PMID:33845085
- 5. Zhixiao Liu, Liujun Wang, et al. "Heterogeneous Responses to Mechanical Force of Prostate Cancer Cells Inducing Different Metastasis Patterns." Adv. Sci. 2020, 1903583.
- 6. Chengcheng Wang, Taiping Zhang, et al. "Metformin inhibits pancreatic cancer metastasis caused by SMAD4 deficiency and consequent HNF4G upregulation." Protein & Cell (2020);31 July 2020. PMID:32737864
